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Título : Operator recognition by the ROK transcription factor family members, NagC and Mlc
Autor(es) : Lenin Dominguez Ramirez, 0000-0002-3483-4658
Autor(es) sin ID: Bréchemier Baey, DominiqueJacques, ObertoPlumbridge, Jacqueline
Fecha de publicación : 2015
Tipo de resultado Científico: article
Materia o Disciplina: BIOLOGÍA Y QUÍMICA
Palabras clave: NagC; Mlc; Proteínas ROK; ADN; N-Acetilglucosamina; Glucosa; Glicina; Arginina
Descripción : NagC and Mlc, paralogous members of the ROK family of proteins with almost identical helix-turn-helix DNA binding motifs, specifically regulate genes for transport and utilization of N-acetylglucosamine and glucose.We previously showed that two amino acids in a linker region outside the canonical helix-turnhelix motif are responsible for Mlc site specificity. In this work we identify four amino acids in the linker, which are required for recognition of NagC targets. These amino acids allowMlc and NagC to distinguish between a C/G and anA/T bp at positions ±11 of the operators. One linker position, glycine in NagC and arginine in Mlc, corresponds to the major specificity determinant for the two proteins. In certain contexts it is possible to switch repression from Mlc-style to NagC-style, by interchanging this glycine and arginine. Secondary determinants are supplied by other linker positions or the helix-turn-helix motif. A wide genomic survey of unique ROK proteins shows that glycine- and arginine-rich sequences are present in the linkers of nearly all ROK family repressors. Conserved short sequence motifs, within the branches of the ROK evolutionary tree, suggest that these sequences could also be involved in operator recognition in other ROK family members.
Editor: Oxford Academic
URI : http://hdl.handle.net/20.500.12222/145
Condiciones de licencia: https://creativecommons.org/licenses/by-nc-nd/4.0/
Fuente: Nucleic Acids Research (1) vol.43 (2015)
ISSN: 1362-4962
Aparece en las colecciones: Artículos Científicos

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