Diacylglycerol acyltransferase 1 inhibition enhances intestinal fatty acid oxidation and reduces energy intake in rats

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dc.audience researchers es_MX
dc.contributor.author Gustavo Pacheco-Lopez, 0000-0002-3458-197X
dc.contributor.other Schober, Gundrun
dc.contributor.other Arnold, Myrtha
dc.contributor.other Birtles, Susan
dc.contributor.other Buckett;, Linda K.
dc.contributor.other Turnbull, Andrew V.
dc.contributor.other Langhans, Wolfgang
dc.contributor.other Mansouri, Abdelhak
dc.coverage MX es_MX
dc.date.accessioned 2018-05-30T18:23:20Z
dc.date.available 2018-05-30T18:23:20Z
dc.date.issued 2013
dc.description Acyl CoA: diacylglycerol acyltransferase-1 (DGAT-1) catalyzes the final step in triacylglycerol (TAG) synthesis and is highly expressed in the small intestine. Because DGAT-1 knockout mice are resistant to diet-induced obesity, we investigated the acute effects of intragastric (IG) infusion of a small molecule diacylglycerol acyltransferase-1 inhibitor (DGAT-1i) on eating, circulating fat metabolites, indirect calorimetry, and hepatic and intestinal expression of key fat catabolism enzymes in male rats adapted to an 8 h feeding-16 h deprivation schedule. Also, the DGAT-1i effect on fatty acid oxidation (FAO) was investigated in enterocyte cell culture models. IG DGAT-1i infusions reduced energy intake compared with vehicle in high-fat diet (HFD)-fed rats, but scarcely in chow-fed rats. IG DGAT-1i also blunted the postprandial increase in serum TAG and increased β-hydroxybutyrate levels only in HFD-fed rats, in which it lowered the respiratory quotient and increased intestinal, but not hepatic, protein levels of Complex III of the mitochondrial respiratory chain and of mitochondrial hydroxymethylglutaryl-CoA synthase. Finally, the DGAT-1i enhanced FAO in CaCo2 (EC50 = 0.3494) and HuTu80 (EC50 = 0.00762) cells. Thus, pharmacological DGAT-1 inhibition leads to an increase in intestinal FAO and ketogenesis when dietary fat is available. This may contribute to the observed eating-inhibitory effect. es_MX
dc.format application/pdf es_MX
dc.identificador.materia 3 es_MX
dc.identifier.uri http://hdl.handle.net/20.500.12222/35
dc.language eng es_MX
dc.publisher Universidad Autónoma Metropolitana. Unidad Lerma es_MX
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.license info:eu-repo/semantics/openAccess es_MX
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ es_MX
dc.subject MEDICINA Y CIENCIAS DE LA SALUD es_MX
dc.subject.keywords Triacilglicerol; es_MX
dc.subject.keywords Cetogénesis; es_MX
dc.subject.keywords Intestino delgado; es_MX
dc.subject.keywords Enterocitos; es_MX
dc.subject.keywords Dieta rica en grasas; es_MX
dc.subject.keywords Alimentación; es_MX
dc.subject.keywords Acil CoA: es_MX
dc.subject.keywords Diacilglicerol aciltransferasa-1Triacylglycerol; es_MX
dc.subject.keywords Ketogenesis; es_MX
dc.subject.keywords Acyl CoA iacylglycerol acyltransferase-1 es_MX
dc.subject.keywords Small intestine; es_MX
dc.subject.keywords Enterocytes; es_MX
dc.subject.keywords Hi es_MX
dc.title Diacylglycerol acyltransferase 1 inhibition enhances intestinal fatty acid oxidation and reduces energy intake in rats es_MX
dc.type preprint es_MX
dc.type.version info:eu-repo/semantics/publishedVersion es_MX
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