Inconsistencies in the hypophagic action of intracerebroventricular insulin in mice

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dc.audience researchers es_MX
dc.contributor.author Gustavo Pacheco-Lopez, 0000-0002-3458-197X
dc.contributor.other Mc Allister, Eugenia
dc.contributor.other Woods, Stephen C.
dc.contributor.other Langhans, Wolfgang
dc.coverage MX es_MX
dc.date.accessioned 2018-06-26T22:49:57Z
dc.date.available 2018-06-26T22:49:57Z
dc.date.issued 2015
dc.description Insulin inhibits eating after its intracerebroventricular (ICV) administration in multiple species and under a variety of conditions. Nevertheless, the results across reports are inconsistent in that ICV insulin does not always reduce food intake. The reasons for this variability are largely unknown. Using mice as a model, we performed several crossover trials with insulin vs. vehicle when infused into the third cerebral ventricle (i3vt) to test the hypothesis that recent experience with the i3vt procedure contributes to the variability in the effect of ICV insulin on food intake. Using a cross-over design with two days between injections, we found that insulin (0.4 μU/mouse) significantly reduced food intake relative to vehicle in mice that received vehicle on the first and insulin on the second trial, whereas this effect was absent in mice that received insulin on the first and vehicle on the second trial. Higher doses (i3vt 4.0 and 40.0 μU/mouse) had no effect on food intake in this paradigm. When injections were spaced 7 days apart, insulin reduced food intake with no crossover effect. Mice that did not reduce food intake in response to higher doses of i3vt insulin did so in response to i3vt infusion of the melanocortin receptor agonist melanotan-II (MT-II), indicating that the function of the hypothalamic melanocortin system, which mediates the effect of insulin on eating, was not impaired by whatever interfered with the insulin effect, and that this interference occurred upstream of the melanocortin receptors. Overall, our findings suggest that associative effects based on previous experience with the experimental situation can compromise the eating inhibition elicited by i3vt administered insulin. es_MX
dc.format application/pdf es_MX
dc.identificador.materia 3 es_MX
dc.identifier.uri http://hdl.handle.net/20.500.12222/151
dc.language eng es_MX
dc.publisher Universidad Autónoma Metropolitana. Unidad Lerma es_MX
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.license info:eu-repo/semantics/openAccess es_MX
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ es_MX
dc.subject MEDICINA Y CIENCIAS DE LA SALUD es_MX
dc.subject.keywords Señales de adiposidad es_MX
dc.subject.keywords Alimentación es_MX
dc.subject.keywords Diseño experimental; es_MX
dc.subject.keywords Péptidos es_MX
dc.subject.keywords Adiposity signals es_MX
dc.subject.keywords Eating es_MX
dc.subject.keywords Experimental design es_MX
dc.subject.keywords Peptides es_MX
dc.title Inconsistencies in the hypophagic action of intracerebroventricular insulin in mice es_MX
dc.type preprint es_MX
dc.type.version info:eu-repo/semantics/publishedVersion es_MX
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