Operator recognition by the ROK transcription factor family members, NagC and Mlc

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dc.audience researchers es_MX
dc.contributor.author Lenin Dominguez Ramirez, 0000-0002-3483-4658
dc.contributor.other Bréchemier Baey, Dominique
dc.contributor.other Jacques, Oberto
dc.contributor.other Plumbridge, Jacqueline
dc.coverage GB es_MX
dc.date.accessioned 2018-06-26T05:27:13Z
dc.date.available 2018-06-26T05:27:13Z
dc.date.issued 2015
dc.description NagC and Mlc, paralogous members of the ROK family of proteins with almost identical helix-turn-helix DNA binding motifs, specifically regulate genes for transport and utilization of N-acetylglucosamine and glucose.We previously showed that two amino acids in a linker region outside the canonical helix-turnhelix motif are responsible for Mlc site specificity. In this work we identify four amino acids in the linker, which are required for recognition of NagC targets. These amino acids allowMlc and NagC to distinguish between a C/G and anA/T bp at positions ±11 of the operators. One linker position, glycine in NagC and arginine in Mlc, corresponds to the major specificity determinant for the two proteins. In certain contexts it is possible to switch repression from Mlc-style to NagC-style, by interchanging this glycine and arginine. Secondary determinants are supplied by other linker positions or the helix-turn-helix motif. A wide genomic survey of unique ROK proteins shows that glycine- and arginine-rich sequences are present in the linkers of nearly all ROK family repressors. Conserved short sequence motifs, within the branches of the ROK evolutionary tree, suggest that these sequences could also be involved in operator recognition in other ROK family members. es_MX
dc.format application/pdf es_MX
dc.identificador.materia 2 es_MX
dc.identifier.other http://dx.doi.org/10.1093/nar/gku1265
dc.identifier.uri http://hdl.handle.net/20.500.12222/145
dc.language eng es_MX
dc.publisher Oxford Academic es_MX
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.license info:eu-repo/semantics/openAccess *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ es_MX
dc.source.other Nucleic Acids Research (1) vol.43 (2015) es_MX
dc.source.other ISSN: 1362-4962 es_MX
dc.subject BIOLOGÍA Y QUÍMICA es_MX
dc.subject.keywords NagC es_MX
dc.subject.keywords Mlc es_MX
dc.subject.keywords Proteínas ROK es_MX
dc.subject.keywords ADN es_MX
dc.subject.keywords N-Acetilglucosamina es_MX
dc.subject.keywords Glucosa es_MX
dc.subject.keywords Glicina es_MX
dc.subject.keywords Arginina es_MX
dc.title Operator recognition by the ROK transcription factor family members, NagC and Mlc es_MX
dc.type article es_MX
dc.type.version info:eu-repo/semantics/publishedVersion es_MX
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