Removal of ammonium and four methyl phenol from synthetic wastewater by cell-free extracts of nitrifying sludge
Removal of ammonium and four methyl phenol from synthetic wastewater by cell-free extracts of nitrifying sludge
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Date
2015
Authors
Ricardo Beristain-Cardoso, 0000-0003-1548-7678
Journal Title
Journal ISSN
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Publisher
Universidad Autónoma Metropolitana. Unidad Lerma
Abstract
Description
The feasibility of using cell-free extracts of nitrifying sludge to treat synthetic wastewater containing 4-methylphenol and ammonium was examined. Nitrifying cells were broken by sonication and encapsulated into calcium alginate. Cell-free extracts (CFE) of nitrifying sludge oxidized 4-methylphenol threefold faster than whole-cells, but CFE were not able to oxidize ammonium. The CFE encapsulated into calcium alginate (CFEA) displayed partial nitrification and 4-methylphenol oxidation. Five hours was enough to oxidize 100 % of ammonium and 4-methylphenol, at volumetric rates of 20.80 mg N/L h and 42.86 mg C/L h, respectively. It is inferred that an interaction between the CFE and calcium alginate resulted in the protection of the enzymes.
Keywords
BIOLOGÍA Y QUÍMICA